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Confocal Imaging Facility

Image Gallery

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mouse motor neuron 

  1. 3D projection from z-stack of mouse motor nerve (green-Cy2) coiling around muscle spindle (not stained)

  mouse muscle bag fiber

  1. 3D projection from z-stack of mouse muscle bag fiber  (red-Cy3) inside capsule (blue-Hoechst) with two motor nerves (green-Cy2)

3-Color separation of 3D projection

  1. 3-Color separation of 3D projection shown above: note degeneration of lower motor nerve (green-Cy2) while upper motor nerve (green-Cy2) looks white where it wraps around muscle bag fiber (red-Cy3).

calcium release time series

  1. Time series tracking calcium release following fertilization of zebra fish egg (false color rendering of calcium green dextran conjugate)

mouse embryo

  1. 3D projection from z-stack of mouse embryo with cell nuclei (blue-Hoechst) and membrane localized protein (red-rhodamine)

fixed cultured cell

  1. Optical section of fixed cultured cell expressing membrane-localized EGFP tagged protein (green)

zebrafish egg, 4-cell stage

  1. 3D projection from z-stack of zebrafish egg, 4-cell stage (green-FITC)

zebrafish egg, 8-cell stage

  1. 3D projection from z-stack of zebrafish egg, 8-cell stage (green-FITC)

3D zebrafish embryo

  1. 3D projection from z-stack of zebrafish embryo (green-FITC)

cultured nerve cell

  1. 3D projection from z-stack of cultured nerve cell (green-FITC) grown on cultured chick embryo muscle with acetylcholine receptor patches (red-Rhodamine).

live cultured cells

  1. 3D projection from z-stack of live cultured cells expressing EGFP tagged protein (green) in Golgi and ER

quantitation of mouse motor nerve

  1. Quantitation of mouse motor nerve endplate volume (red-Rhodamine) with 3DLSM software

mouse kidney profile analysis

  1. Optical section of mouse kidney stained for two different basement membrane laminins (green-FITC and red-TRITC) showing co-localization (yellow) and quantitation by peak intensity profile analysis

live cultured cell quantitation

  1. Optical section of live cultured cell expressing membrane-localized EGFP tagged protein (green) and quantitation by 2.5D software

mouse hindbrain

  1. Optical section of mouse hindbrain showing normal cytoplasmic localization of a steroid receptor (top, red-Cy3 and blue-DAPI) and nuclear localization of the receptor (bottom) following experimental treatment

cultured nerve cells

  1. Optical section of cultured nerve cells with nuclei (blue-Hoechst), cytoskeletal marker (green-FITC) and cytokine label (red-TRITC)