1979-1984: Shevchenko National University, Kiev, Ukraine, M.S., Physics.
1984-1989: Institute of Biochemistry, NANU, Kiev, Ukraine, Ph.D., Biophysics.
1990-1992: University of Virginia, Charlottesville, VA, Postdoctoral Fellow.
1992-1994: The Johns Hopkins University, Baltimore, MD, Postdoctoral Fellow.
1994-2004: University of California, Irvine, CA, Research Scientist
2004-2008: University of Kansas Medical Center, Kansas City, KS, Department of
Biochemistry and Molecular Biology, Assistant Professor
2008-present: University of Kansas Medical Center, Kansas City, KS, Department of
Biochemistry and Molecular Biology, Associate Professor
PubMed References for A.S. Ladokhin
Major Research Interest
I. Structure, Folding and Functioning of Membrane Proteins
This research is driven by a search for answers to two questions: how do proteins cross membranes, and how do they insert into lipid bilayers? In the cell, these vital functions are achieved by complex multi-protein assemblies. But the diphtheria toxin T-domain by itself inserts into the membrane under acidic conditions and translocates the catalytic domain across the lipid bilayer. Low pH is also involved in transbilayer insertion of annexin 12, a representative of a large protein family that has been implicated in a variety of membrane functions and in a number of human diseases. The pH-triggered refolding-insertion-translocation of the T-domain and other proteins (e.g. botulinum toxin) is not only of inherent importance, but also can reveal general physicochemical principles underlying membrane protein assembly and stability. (Funded by NIH)
II. Ion Channels, Blockers
III. Antimicrobial Peptides
IV. Translocon-Assisted Membrane Insertion, Sorting
V. Biophysical Methods, Single-Molecule Spectroscopy
Ladokhin Lab, July, 2008
Recent Publications of Alexey S. Ladokhin
Posokhov, Y.O., Merzlyakov, M., Hristova, K. and Ladokhin, A. S. A simple "proximity" correction for Förster resonance energy transfer efficiency determination in membranes using lifetime measurements, Analytical Biochem. In press (2008).
Rodnin, M.V., Posokhov, Y.O., Contino-Pepin, C., Brettmann, J., Kyrychenko, A., Palchevskyy, S.S.. Pucci, B., and Ladokhin, A. S. Interactions of fluorinated surfactants with diphtheria toxin T-domain: Testing new media for studies of membrane proteins. Biophysical Journal, 94, 4348-57 (2008).
Posokhov, Y.O., Rodnin, M.V., Lu, L. and Ladokhin, A. S. Membrane Insertion Pathway of Annexin B12: Thermodynamic and Kinetic Characterization by FCS and Fluorescence Quenching. Biochemistry 47, 5078-87 (2008).
Ladokhin, A. S. Insertion Intermediate of Annexin B12 is Prone to Aggregation on Membrane Interfaces. Biopolymers and Cell, 24(2):100-104 (2008).
Posokhov, Y.O., Gottlieb, P.A., Morales, M.J., Sachs, F. and Ladokhin, A. S. Is Lipid Bilayer Binding a Common Property of Inhibitor Cysteine Knot Ion-Channel Blockers? Biophysical Journal 93(4):L20-2 (2007).
Fernandez-Vidal M., Jayasinghe S., *Ladokhin A.S., *White S.H. Folding Amphipathic Helices Into Membranes: Amphiphilicity Trumps Hydrophobicity, J. Mol. Biol. 370(3):459-70 (2007).
Posokhov, Y.O., Gottlieb, P.A. and Ladokhin, A. S. Quenching-enhanced fluorescence titration protocol for accurate determination of free energy of membrane binding, Analytical Biochem. 362, 290-292 (2007).
Palchevskyy, S.S, Posokhov, Y.O., Olivier, B., Popot, J-L, Pucci, B. and Ladokhin, A. S. Chaperoning of insertion of membrane proteins into lipid bilayers by hemifluorinated surfactants: application to diphtheria toxin Biochemistry 45, 2629-2635 (2006).
Posokhov, Y.O. and Ladokhin, A. S. Lifetime Fluorescence Method for Determining Membrane Topology of Proteins, Analytical Biochem. 348, 87-93 (2006).
Ladokhin, A. S. and Haigler, H. T. Reversible Transition between the Surface Trimer and Membrane-Inserted Monomer of Annexin 12. Biochemistry 44, 34042-3409 (2005).
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