Biotechnology Support Facility

Mission

The Biotechnology Support Facility falls under the auspices of the Department of Biochemistry and Molecular Biology. The mission of the Biotechnology Support Facility is to “Advance the Science of KUMC Investigators” by

1. Providing KUMC investigators with state-of-the-art technologies, methodologies, and instrumentation in a centralized facility that enhances their research.
2. Freeing KUMC investigators to intellectually advance their science by performing laborious bench work with automated instrumentation.
3. Saving KUMC investigators valuable research time by offering a productivity and performance level that provides superior products and data in less time than they can provide themselves.
4. Serving KUMC investigators as an academic resource center for edification in the technology, methodology, and instrumentation of a modern biotechnology core facility.

Description of Services

• DNA Sequencing - Order Form
• Oligonucleotide Synthesis - Order form
  Service Pricing
• GeneChip Microarray Analysis - Order form

DNA Sequencing

DNA sequencing is performed using an Applied Biosystems 3130XL Genetic Analyzer. The system utilizes dye terminator fluorophores to sequence single or double stranded plasmid templates, BAC templates, or PCR fragments. The contiguous sequence of DNA bases is generated using proprietary  base calling software. To enhance sequencing results, the facility recommends Qiagen or similar cartridge purified templates and purified custom primers. Some standard sequencing primers are provided by the facility at no additional cost.

Oligonucelotide Synthesis

Oligonucleotide synthesis is performed on an Applied Biosystems 3900 High Throughput DNA Synthesizer. Synthesis is accomplished on controlled pore glass supports in a 3' to 5' direction. Scale of synthesis on the 3900 is 40-50 nanomole, 200 nanomole, or 1 micromole. The BSF recommends purified oligonucleotides where the purity of the primer may enhance the investigator's results. Purification of oligonucleotides is performed on single-use trityl specific cartridges.

GeneChip Microarray Analysis

For a full description of services provided by the Microarray Facility, please go to their webage here. For information regarding genechip microarray analysis please contact Clark Bloomer at Tel: (913)588-7127 or by E-mail: at cbloomer@kumc.edu.

Staff

• Director: Dr. Gerald M. Carlson, Chairperson Biochemistry and Molecular Biology
• Sr. Research Associate: Jim Rouse, Lab Manager
• Research Associate: Rosanne Skinner
• Project Manager: Clark Bloomer, Microarray Facility

For Further Information Contact

Jim Rouse
Biotechnology Support Facility
1015 KLSIC
University of Kansas Medical Center, (KUMC)
2146 West 39th Street
Kansas City, KS 66160
Phone: (913) 588-7052
FAX: (913) 588-7131
E-mail: jrouse@kumc.edu

or

Roseanne Skinner
Biotechnology Support Facility
1015 KLSIC
University of Kansas Medical Center, (KUMC)
2146 West 39th Street
Kansas City, KS 66160
Phone: (913) 588-6927
FAX: (913) 588-7131
E-mail: rskinner@kumc.edu

or

Clark Bloomer
Microarray Facility
1015 KLSIC
University of Kansas Medical Center, (KUMC)
2146 West 39th Street
Kansas City, KS 66160
Phone: (913) 588-7127
FAX: (913) 588-7131
E-mail: cbloomer@kumc.edu