Biotechnology Support Facility

Mission

The Biotechnology Support Facility falls under the auspices of the Department of Biochemistry and Molecular Biology. The mission of the Biotechnology Support Facility is to “Advance the Science of KUMC Investigators” by

1. Providing KUMC investigators with state-of-the-art technologies, methodologies, and instrumentation in a centralized facility that enhances their research.
2. Freeing KUMC investigators to intellectually advance their science by performing laborious bench work with automated instrumentation.
3. Saving KUMC investigators valuable research time by offering a productivity and performance level that provides superior products and data in less time than they can provide themselves.
4. Serving KUMC investigators as an academic resource center for edification in the technology, methodology, and instrumentation of a modern biotechnology core facility.

Available Services

Downloadable order forms for the offered services can be obtained by double clicking on the words “Order form” which are next to the listed services. The order forms are .PDF files which can be filled out online using Adobe Acrobat Reader. If you have problems completing the form online, then you may print the file and fill it out manually. Please contact the facility directly, Tel:(913) 588-6927, E-mail: jrouse@kumc.edu or rskinner@kumc.edu for information regarding our DNA services. For information regarding microarray gene expression analysis please contact Clark Bloomer at Tel: (913)588-7127 or by E-mail: at cbloomer@kumc.edu.

• DNA Sequencing - Order Form
• Oligonucleotide Synthesis - Order form
  Service Pricing
• Microarray Gene Expression Analysis - Order form
  
  

Description of Services

DNA Sequencing

DNA sequencing is performed using an Applied Biosystems 3130XL Genetic Analyzer. The system utilizes dye terminator fluorophores to sequence single or double stranded plasmid templates, BAC templates, or PCR fragments. The contiguous sequence of DNA bases is generated using proprietary  base calling software. To enhance sequencing results, the facility recommends Qiagen or similar cartridge purified templates and purified custom primers. Standard sequencing primers are provided by the facility at no additional cost.

Oligonucelotide Synthesis

Oligonucleotide synthesis is performed on an Applied Biosystems 3900 High Throughput DNA Synthesizer. Synthesis is accomplished on controlled pore glass supports in a 3' to 5' direction. Scale of synthesis on the 3900 is 40-50 nanomole, 200 nanomole, or 1 micromole. The BSF recommends purified oligonucleotides where the purity of the primer may enhance the investigator's results. Purification of oligonucleotides is performed on single-use trityl specific cartridges.

Microarray Gene Expression Analysis

For a full description of services provided by the Microarray Facility, please go to their webage here.

Staff

• Director: Dr. Gerald M. Carlson, Chairperson Biochemistry and Molecular Biology
• Lab Manager: Bo Wisdom, M.A.
• Research Associates: Jim Rouse and Clark Bloomer
• Research Assistant: Rosanne Skinner

For Further Information Contact

Jim Rouse
Biotechnology Support Facility
1015 KLSIC
University of Kansas Medical Center, (KUMC)
2146 West 39th Street
Kansas City, KS 66160
Phone: (913) 588-7052
FAX: (913) 588-7131
E-mail: jrouse@kumc.edu

or

Clark Bloomer
Microarray Facility
1015 KLSIC
University of Kansas Medical Center, (KUMC)
2146 West 39th Street
Kansas City, KS 66160
Phone: (913) 588-7127
FAX: (913) 588-7131
E-mail: cbloomer@kumc.edu