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Transgenic & Gene-targeting Institutional Facility
Transgenic & Gene-targeting Institutional Facility  :  Services, Forms & Pricing   :  Transgenic Mouse Production

Transgenic Mouse Production

Services provided by the facility

  • Use mouse strain C57B6/SJL as default unless requested by the user and approved by the facility.
  • The facility will superovulate donor mice, harvest single cell embryos and inject DNA provided by the investigator.  Surviving embryos will be implanted into pseudopregnant females.
  • Plasmid DNA will be provided to the facility were it will be linearized and purified.  The DNA will then be used for injected into embryos and implanted. Genotyping will also be provided by the facility to identify transgenic founders.  A guarantee of at least two transgenic founders will be provided for each construct. 

Responsibilities of the investigator

  1. Discuss experimental design with facility director before initiation of the project. Important, when designing your vector, ensure the transgene sequence is flanked by unique restriction enzyme sites. This allows for easy removal of vector backbone (if left on, the vector backbone sequence can interfere with expression of your transgene) when preparing the DNA for injection.
  2. Provide 25ug of your circular DNA construct at a concentration of 1ug/ul in TE.
  3. Provide a map and sequence of the construct including size, vector, promoter, gene of interest and/or reporter gene.
  4. Provide gel documentation of the purity and concentration of the DNA construct.
  5. Obtain IACUC approval for your project before submission of the DNA.
  6. Obtain space within the Animal Resources Center for housing founder animals.
  7. Submit a “service request form”.

 Fees for Gene Targeted Mouse service

  • $3000 will be charged upon identifying 2 founder mice.
  • $2500 when using BAC or YAC DNA. IMPORTANT. Currently we are unable to prep BAC or YAC DNA for investigators. We can still inject these types of DNA if provided by the investigator using an approved purification protocol. In addition, we will only guarentee the injection of 200 embryos, not 2 founders as stated with plasmid DNA. Finally, it will be the investigators responsiblity to genotype the mice.
  • Service Request Form for Transgenic Mouse Production.

 Service Request