Genotyping

Services provided by the facility

• The core will prep genomic DNA from tail biopsies using the REDExtract-N-Amp Tissue PCR kit (Sigma, cat # XNATS).
• The facility will perform a PCR for detecting the transgene(s) of interest. For an additional fee, a second PCR (GDPH, a housekeeping gene) to analyze the quality of genomic DNA can be performed.

Responsibilities of the investigator

• Submit “Requisition Form” for services to be rendered.
• Provide the tail biopsies uniquely labeled and the plasmid used to generate the transgenics (used for a positive control).
• Provide a proven PCR assay protocol and primers (100ul at 10uM concentration).

Fees for Genotyping service

• $5.00/biopsy for genomic DNA preparation and PCR analysis (a minimum of $20.00 per requisition).  Any additional PCR analysis will be charged an additional $2.50/sample. If a proven PCR assay is not provided, an additional fee of $100.00 will be charged to optimize a suitable PCR assay.
• Service Request Form for Genotyping.

Click here for the requisition form for Genotyping