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Transgenic & Gene-targeting Institutional Facility
Transgenic & Gene-targeting Institutional Facility  :  Services, Forms & Pricing   :  ES Cell Targeting

ES Cell Targeting

Services provided by the facility

  • The gene-targeting facility will electroporate DNA provided by the investigator into embryonic stem cells (ES cells) and perform either positive or positive-negative selection.
  • ES cells lines provided:
      • E14 (129/Ola embryonic stem cell)
      • B6-albino (C57BL/6J albino embryonic stem cell)
      • CJ7 (129S1/Sv embryonic stem cell)
      • W4 (129S6 embryonic stem cell)
  • Genomic DNA from surviving clones will be returned to the investigator to assay for targeted clones. Guaranteed 100+ clones for positive-negative selection and 300+ clones for positive selection or perform up to 3 electroporations for each targeting construct.
  • The basic service will expand and freeze all "positive clones" and karyotype 4 of these lines for the set price listed below. Any additional expanding and/or karyotyping of clones will be subject to an additional charge listed below.

Responsibilities of the investigator

  • Discuss experimental design with Melissa Larson (TGIF director) before beginning a project.
  • Provide a map of the construct including size, regions of homology, selection strategy, enzyme sites, and oligo binding sites.
  • Purify the DNA targeting vector according to protocols provided by the facility. We’ll need 50ug of purified, linearized DNA at ~1ug/ul.
  • Provide gel documentation of the purity and concentration of the DNA target.
  • Develop a reliable genotyping assay (long PCR, Southern, or both) before submission of DNA to the facility.
  • Submit “Requisition Form” for services to be rendered.
  • Storage of frozen ES cells for three months while the DNA is being analyzed by the investigator.

Fees for ES Cell Targeting service

  • $4400.00 ES cell targeting (includes expansion and karyotyping of screened, selected ES cells, see above for limit).
  • $220.00 additional ES cell karyotyping.
  • $330.00 additional ES cell expansion.
  • Service Request Form for ES Cell Targeting

Click here for the requisition form for Gene Knockout in ES cells