Sperm Cryopreservation

Services provided by the facility

• Freeze and store 5 vials in liquid nitrogen. 
• Thaw sperm within 7 days to record post-freezing viability (%) and motility (+, ++, +++).
• Record (pre- and post-freezing) sperm viability and motility.
• Males sperm will be deemed acceptable if fresh sperm has a < 40% viability and “++ or +++” motility or thawed sperm has a < 20% viability and “++ or +++” motility.

Responsibilities of the investigator

• Provide the facility with one male (2-4 months old) for each line.
• Submit a “service request form” asking for strain, age, and successful mating record.
• May be asked to supply a second male if first submitted male sperm is unacceptable.
• Even though we request a 2-4 month old male, we can process any strain or age of mouse; however, it should be noted that age and mouse strain can affect the viability, motility, and recovery quality of the sperm.

Fees for Gene Targeted Mouse service

• $165.00 for a successful cryopreservation per line.
• A yearly storage fee of $50.00 will be charged for 1-50 vials of targeted ES cells, $100.00 for 51-100 vials, and $150.00 for 101-150 vials.
• Service Request Form for Sperm Cryopreservation.

Click here for the requisition form for Mouse Sperm Cryopreservation