Embryo Cryopreservation

Services provided by the facility

Embryo cryopreservation will be accomplished by mating donor female mice to stud males of the line of interest. The facility can purchase wildtype females of either outbred or inbred background, or the investigator can provide the donor females for a reduced fee. (Required if cryopreserving homozygous mice.)

• Superovulate donor female mice and mate to investigator stud males.
• Euthanize donors and harvest embryos.
• Freeze embryos in straws with a controlled rate freezer. 
• Store straws in liquid nitrogen.
• Thaw test straw to confirm viability of embryos.
• Guarantee 250 embryos frozen or three freezing sessions.

Responsibilities of the investigator

• Have a minimum of five proven stud males available for each line.
• If providing female mice, donors must be between three and four weeks of age, provided in groups of at least five.
• Submit a requisition for Embryo Cryopreservation..

Fees for Embryo Cryopreservation service

• $1500 if investigator provides donor females.
• $2000 if facility purchases outbred donor females.
• $2500 if facility purchases inbred donor females.
• Service Request Form for Embryo Cryopreservation.

Click here for the requisition form for Mouse Sperm Cryopreservation