Welcome to the Transgenic and Gene-targeting Facility at the University of Kansas Medical Center! We are an institutional support facility providing embryo, embryonic stem cell and transgenic services to the investigators of KUMC and the surrounding Kansas City research community.
Currently, our facility can generate transgenic mice by pronuclear microinjection, chimeric mice by blastocyst injection with ES cells and modified embryonic stem cells by gene targeting. We can also provide services in sperm cryopreservation, rederivation by embryo transfer and genotyping by PCR.
In addition, the Transgenic Facility will be moving to new laboratory space in the Fall of 2007 in the newly constructed Kansas Life Sciences Innovative Center on the University of Kansas Medical Center campus. Coincident with this move, the facility will be purchasing another Nikon TE2000 inverted microscope equipped with DIC, HMC and fluorescence, as well as Eppendorf Cell Tram air and vario micrometers, an Eppendorf Femtojet and a Piezo drill.
Other new equipment purchases include four Sanyo MCO-18M tri-gas incubators; new stereo and tissue culture microscopes with video and high resolution camera capabilities; a new controlled-rate freezer; an auto-fill liquid nitrogen storage tank and a vapor shipper. The facility will then be equipped to offer a full range of embryo services, including generation of transgenics, chimeras and targeted embryonic stem cells; cryopreservation of sperm, embryos and oocytes; and assisted reproductive techniques such as rederivation, in vitro fertilization, and intracytoplasmic sperm injection.
The new laboratory space will centralize our operations, with accommodations for embryo manipulations and cryogenics, distinct cell culture space, animal holding rooms and offices. We will also be equipped to provide training and demonstrations in embryo, ESC and mouse surgical techniques.
Investigators are invited to peruse the links at the left for detailed information on services and fees. Please submit the appropriate requisition form when requesting service, and please feel free to stop by or give us a call if you have questions. We welcome the opportunity to facilitate your research… if it involves embryos, we can do it!
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The University of Kansas Medical Center